Characterization of beta-lactamases in situ on polyacrylamide gels

Demonstration of rhodanese activity in polyacrylamide gels.

Rhodanese activity from crude extracts of Thiobacillus sp. strain IV-85 was demonstrated in polyacrylamide gels after incubation in the reaction mixture by staining with dichloroindophenol in the presence of methylphenazonium methosulfate. The sensitivity of the staining system was found to be 8 x 10 moles of sulfite.

Improved recovery of DNA from polyacrylamide gels after in situ DNA footprinting.

Methods used to date for the isolation of DNA from polyacrylamide gels are elution based, time-consuming and with low yield in DNA. This paper describes an improved system employing polyacrylamide gels made of a meltable matrix. The new system was successfully applied to in situ DNA footprinting following gel retardation assays.

In situ detection method for glutaminyl cyclase activity in polyacrylamide gels.

that achieved by TLC with numerous buffers (1,2,5) or by HV-TLE protocols (2) when multiple samples are analyzed. We have found that when 1 μL of a dye solution (1% orange G, 1% phenol red in pH 3.5 buffer) was electrophoresed in parallel with the phosphoamino acid standards, orange G migrated slightly ahead of pSer and phenol red migrated with pTyr, providing useful visible markers for electro...

Steps toward peptide polymerization in polyacrylamide gels

Microfluidic 2-D PAGE using multifunctional in situ polyacrylamide gels and discontinuous buffers.

A two-dimensional microfluidic system is presented for intact protein separations combining isoelectric focusing (IEF) and sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) employing in situ photopolymerized polyacrylamide (PAAm) gels. The PAAm gels are used for multiple functions. In addition to serving as a highly-resolving separation medium for gel electrophoresis, discr...